MUC5AC anticorps (CF®647)

N° du produit ABIN6186124

L’anticorps Souris Monoclonal anti-MUC5AC a été validé pour IF, FACS et IHC (f). Il convient pour détecter MUC5AC dans des échantillons de Humain, Souris, Rat, Singe, Cat, Lapin, Poulet, Porc et Hedgehog.

Aperçu rapide pour MUC5AC anticorps (CF®647) (ABIN6186124)

Antigène: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Reactivité: Humain, Souris, Rat, Singe, Cat, Lapin, Poulet, Porc, Hedgehog

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MUC5AC est conjugé à/à la CF®647

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 45M1

Détail du produit anti-MUC5AC anticorps (CF®647)

Fonction: Mouse Monoclonal anti-Mucin 5AC (MUC5AC) (45M1), CF647 Conjugate

Attributs du produit: This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC). Its epitope is located in the C-terminal cysteine rich part of the peptide core of MUC5AC. TheA,  epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 1-2 μg/mL

• Does not react with cow, others not known
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 1-2 μg/mL
• Flow Cytometry 0.5-1 μg/million cells in 0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Stomach

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur MUC5AC

Antigène: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Autre désignation: Mucin 5AC (MUC5AC)

Sujet: Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM)

Poids moléculaire: >1,000 kDa

ID gène: 4586, 534332

UniProt: P98088